9GCX

Crystal structure of bovine Cytochrome bc1 in complex with inhibitor F8

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 65 2 2
Unit cell lengths	210.082, 210.082, 344.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.522
Rwork	0.188
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	2.081
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.650	3.620
High resolution limit [Å]	3.520	3.540
Rmerge	0.150
Rpim	0.050	0.510
Number of reflections	55657	3699
<I/σ(I)>	13.5	1.6
Completeness [%]	99.6	96.5
Redundancy	10.3
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	50 mM KPi pH 6.8, 100 mM NaCl, 3 mM NaN3, 10-20% PEG4000