9GCX
Crystal structure of bovine Cytochrome bc1 in complex with inhibitor F8
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 210.082, 210.082, 344.844 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.522 |
| Rwork | 0.188 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.081 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.650 | 3.620 |
| High resolution limit [Å] | 3.520 | 3.540 |
| Rmerge | 0.150 | |
| Rpim | 0.050 | 0.510 |
| Number of reflections | 55657 | 3699 |
| <I/σ(I)> | 13.5 | 1.6 |
| Completeness [%] | 99.6 | 96.5 |
| Redundancy | 10.3 | |
| CC(1/2) | 0.990 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 50 mM KPi pH 6.8, 100 mM NaCl, 3 mM NaN3, 10-20% PEG4000 |
