9GBH

CRYSTAL STRUCTURE OF HUMAN CHYMASE IN COMPLEX WITH COMPOUND1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-04-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.541870
Spacegroup name	P 43
Unit cell lengths	74.093, 74.093, 49.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.040 - 2.375
Rwork	0.202
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.584
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.040	2.490
High resolution limit [Å]	2.360	2.360
Rmerge	0.113	0.270
Number of reflections	574082	18908
<I/σ(I)>	4.5929
Completeness [%]	97.5
Redundancy	13.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.01M ZnCl2, 0.1M TRIS at pH 8 and 20% PEG 6000