9GAU

Sumo-Darpin-C10-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999
Spacegroup name	C 1 2 1
Unit cell lengths	90.461, 44.687, 47.602
Unit cell angles	90.00, 98.76, 90.00

Refinement procedure

Resolution	47.050 - 2.640
R-factor	0.2385
Rwork	0.233
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.589
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.050	2.770
High resolution limit [Å]	2.640	2.640
Rmerge	0.293	1.614
Rmeas	0.329	1.798
Rpim	0.144	0.776
Total number of observations		3612
Number of reflections	5539	695
<I/σ(I)>	6.5	1.6
Completeness [%]	98.5
Redundancy	5	5.2
CC(1/2)	0.974	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Bis-Tris. 16% (v/v)PEG 3350