Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979999 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.596, 81.601, 46.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.720 - 1.400 |
| R-factor | 0.15689 |
| Rwork | 0.156 |
| R-free | 0.18250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.425 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.000 | 65.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 4.190 | 1.400 |
| Rmeas | 0.073 | 0.028 | 2.302 |
| Number of reflections | 60722 | 2467 | 9648 |
| <I/σ(I)> | 18.31 | 77.44 | 1.17 |
| Completeness [%] | 99.9 | 99.9 | 99.6 |
| Redundancy | 13.27 | 12.92 | 13.19 |
| CC(1/2) | 1.000 | 1.000 | 0.588 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M lithium sulfate, 0.1 M TRIS pH 8.5, PEG 4000 20%, 3 mM sodium azide |
