9GA8
The crystal structure of human Annexin A4 from crystals grown at 4 mM Calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.88560 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.613, 39.620, 73.496 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 58.377 - 1.500 |
| Rwork | 0.202 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.896 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.200 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.037 | |
| Number of reflections | 52824 | 2410 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 96.8 | |
| Redundancy | 2.7 | |
| CC(1/2) | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1 M sodium acetate (pH 4.6), 0.2 M ammonium sulfate, and 25 % (v/v) PEG 4000 |
