9GA7
The crystal structure of human Annexin A4 derived from crystal grown at 4 mM CaCl2 and retro-soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2002-07-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.384, 39.926, 65.904 |
| Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
| Resolution | 65.076 - 1.446 |
| Rwork | 0.186 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.787 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.100 | 1.470 |
| High resolution limit [Å] | 1.400 | 1.450 |
| Rmerge | 0.048 | 0.495 |
| Number of reflections | 51347 | 2372 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 95.3 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M sodium acetate (pH 4.6), 0.2 M ammonium sulfate, and 25 % (v/v) PEG 4000 |
