9GA6
The crystal structure of human Annexin A4 derived from crystals grown in 40 mM of CaCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-21 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.991874 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.742, 39.906, 73.456 |
| Unit cell angles | 90.00, 93.91, 90.00 |
Refinement procedure
| Resolution | 58.600 - 1.270 |
| Rwork | 0.174 |
| R-free | 0.18910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.896 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.600 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.059 | 0.600 |
| Rmeas | 0.089 | |
| Rpim | 0.059 | |
| Number of reflections | 86437 | 4117 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 95.8 | 91.7 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M sodium acetate (pH 4.6), 0.2 M ammonium sulfate, and 25 % (v/v) PEG 4000. |
