9G9U
The structure of XynX, a NIF3 family protein from Geobacillus proteiniphilus T-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-04 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.586, 58.301, 99.115 |
| Unit cell angles | 90.00, 99.73, 90.00 |
Refinement procedure
| Resolution | 43.210 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.953 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.210 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.790 |
| Rmeas | 0.094 | 0.830 |
| Rpim | 0.028 | 0.240 |
| Number of reflections | 411459 | 2667 |
| <I/σ(I)> | 15.9 | 3 |
| Completeness [%] | 92.0 | |
| Redundancy | 11.2 | |
| CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% PEG 3350 and 0.1M Citric acid with Bis-Tris propane pH 7.6. |
