9G9O
Lipid III flippase WzxE with NB10 and NB7 nanobodies in outward-facing conformation - crystal 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-28 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 0.6702 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.335, 154.019, 288.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.500 - 2.690 |
| R-factor | 0.2822 |
| Rwork | 0.279 |
| R-free | 0.33780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.451 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.270 | 2.730 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 26959 | 1293 |
| <I/σ(I)> | 4.1 | 0.4 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 293 | sodium citrate, lithium sulfate, sodium chloride, PEG300, E. coli polar lipids, monoolein |
