9G95
Lipid III flippase WzxE with NB10 nanobody in outward-facing conformation at 2.7552 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-07-26 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.7552 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 271.540, 38.450, 56.960 |
| Unit cell angles | 90.00, 93.09, 90.00 |
Refinement procedure
| Resolution | 44.780 - 2.800 |
| R-factor | 0.2383 |
| Rwork | 0.236 |
| R-free | 0.28870 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.658 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.190 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 14932 | 2147 |
| <I/σ(I)> | 30.7 | 2.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 85.1 | 19.6 |
| CC(1/2) | 0.999 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | Na acetate, PEG400, lithium sulfate, zinc chloride, E. coli polar lipids, monoolein |
