9G7U

Structure of the Tesup-1 proline-rich binding domain in complex with the proline-rich region of Pih1d1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.87313
Spacegroup name	I 2 2 2
Unit cell lengths	41.387, 75.087, 112.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.540 - 2.100
R-factor	0.2504
Rwork	0.250
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.853
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.370	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.037
Number of reflections	10539	862
<I/σ(I)>	13.7
Completeness [%]	99.6
Redundancy	13.3
CC(1/2)	1.000	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.2M Magnesium chloride hexahydrate, 0.1 M Tris pH 8.0, 20% w/v PEG 6000