9G7N
Crystal structure of Chromobacterium haemolyticum PE-like toxin, Hmx
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-01 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.325, 76.019, 130.493 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.686 - 1.350 |
| Rwork | 0.137 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.486 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.690 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.062 | 1.538 |
| Rmeas | 0.067 | 1.856 |
| Rpim | 0.026 | 1.013 |
| Number of reflections | 143804 | 6411 |
| <I/σ(I)> | 16.3 | 0.7 |
| Completeness [%] | 99.1 | 90.8 |
| Redundancy | 11.8 | 5.7 |
| CC(1/2) | 1.000 | 0.437 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294.15 | 0.12 M Monosaccharides, 0.1 M Buffer System 1 pH 6.5, 37.5 % v/v Precipitant Mix 4 (condition F4 of the Morpheus Screen, Molecular Dimensions) |
