9G7L
Structure of the proline-rich binding domain of Tesup-1 in complex with dZfc3h1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-30 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.568, 48.625, 66.579 |
| Unit cell angles | 90.00, 113.04, 90.00 |
Refinement procedure
| Resolution | 61.270 - 1.900 |
| R-factor | 0.2178 |
| Rwork | 0.216 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.492 |
| Data reduction software | XDS |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.320 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rpim | 0.041 | |
| Number of reflections | 30043 | 1397 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.997 | 0.304 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.1 M Sodium acetate pH 4.6, 1.1 M Ammonium Tartrate |
