9G7E
Crystal structure of ASGPR with bound guanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.868, 32.554, 40.583 |
| Unit cell angles | 90.00, 92.29, 90.00 |
Refinement procedure
| Resolution | 56.390 - 1.398 |
| R-factor | 0.2048 |
| Rwork | 0.204 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.830 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.390 | 1.594 |
| High resolution limit [Å] | 1.398 | 1.398 |
| Rmerge | 0.101 | 0.876 |
| Number of reflections | 15814 | 786 |
| <I/σ(I)> | 8.9 | 1.3 |
| Completeness [%] | 53.7 | 8.3 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PROTEIN BUFFER: 20 MM TRIS-HCL (PH 7.4), 25 MM CACL2. RESERVOIR: 100 MM TRIS-HCL (PH 7.5), 28% PEG4000, PH 7.50, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
