9G6Z

C. elegans TOFU-6 eTUDOR TOFU-1 peptide complex

Replaces:  8BY5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-26
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9677
Spacegroup name	C 1 2 1
Unit cell lengths	59.150, 69.202, 60.946
Unit cell angles	90.00, 91.47, 90.00

Refinement procedure

Resolution	36.520 - 2.220
R-factor	0.181
Rwork	0.176
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.671
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.930	2.260
High resolution limit [Å]	2.220	2.220
Number of reflections	12063	626
<I/σ(I)>	10.3
Completeness [%]	99.0
Redundancy	5.1
CC(1/2)	0.992	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.2 M Na bromide, 0.1 M Bis Tris propane pH 7.5, 20% (w/v) PEG 3350