9G64

F420-dependent oxidoreductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	56.143, 94.793, 111.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.310 - 1.650
Rwork	0.170
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.310	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.120	1.750
Number of reflections	71666	3528
<I/σ(I)>	19.4
Completeness [%]	99.6
Redundancy	26.8
CC(1/2)	0.990	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% (w/v) PEG 4000, 0.2 M Sodium acetate and 0.1 M Tris-Cl pH 8.5