9G5G
Glycoside Hydrolase Family 157 from Labilibaculum antarcticum (LaGH157) in complex with Laminaribiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-12 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | H 3 |
| Unit cell lengths | 208.628, 208.628, 165.285 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 121.950 - 2.710 |
| R-factor | 0.21388 |
| Rwork | 0.211 |
| R-free | 0.27230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.623 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.950 | 2.770 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.166 | 1.894 |
| Rmeas | 0.182 | 2.086 |
| Rpim | 0.074 | 0.860 |
| Total number of observations | 429854 | 21966 |
| Number of reflections | 71618 | 3895 |
| <I/σ(I)> | 9.6 | 0.8 |
| Completeness [%] | 98.6 | |
| Redundancy | 6 | 5.6 |
| CC(1/2) | 0.995 | 0.375 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Sodium malonate, 10% PEG3350 |
