9G4R

Crystal structure of the DUF2693-FD RNA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 65 2 2
Unit cell lengths	110.761, 110.761, 74.784
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.960 - 2.530
R-factor	0.187
Rwork	0.185
R-free	0.21600
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.256
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.961	2.570
High resolution limit [Å]	2.530	2.530
Rmerge	0.053	0.800
Rpim	0.021	0.315
Number of reflections	16698	470
<I/σ(I)>	17.7
Completeness [%]	100.0	100
Redundancy	7.1	7.3
CC(1/2)	0.999	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	0.1M Sodium chloride 0.1M Potassium chloride 0.05M HEPES6.5 1.9M Lithium sulfate