9G4Q

Crystal structure of the salivarius-1 RNA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9198
Spacegroup name	P 21 21 21
Unit cell lengths	49.409, 68.094, 99.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.240 - 1.990
R-factor	0.226
Rwork	0.225
R-free	0.25500
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	1.020
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.170	2.020
High resolution limit [Å]	1.990	1.990
Rmerge	0.109	3.504
Rpim	0.022	0.697
Number of reflections	44110	1125
<I/σ(I)>	15.6
Completeness [%]	100.0	98.51
Redundancy	25.3	25.8
CC(1/2)	1.000	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	290	0.04 M Magnesium Acetate, 0.05 M Sodium Cacodylate pH 6.0, 30% v/v 2-Methyl-2,4-pentanediol