9G4P

Crystal structure of the skipping-rope RNA motif.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9196
Spacegroup name	P 61
Unit cell lengths	113.478, 113.478, 59.286
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.830 - 2.900
R-factor	0.195
Rwork	0.193
R-free	0.23200
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.822
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.280	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.095	1.091
Rpim	0.022	0.247
Number of reflections	18131	473
<I/σ(I)>	16.48
Completeness [%]	100.0	100
Redundancy	19.74	20.1
CC(1/2)	1.000	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	0.001M Spermine tetrahydrochloride 0.05M MES6.5 30% v/v PEG 400