9G43
Crystal structure of a galactose oxidase from Pseudoarthrobacter siccitolerans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.271, 62.943, 183.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.640 - 1.200 |
| R-factor | 0.13532 |
| Rwork | 0.134 |
| R-free | 0.16609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.656 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.640 | 1.290 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 138716 | 6936 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 92.6 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.997 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M sodium HEPES and MOPS pH 7.5, 0.03 M magnesium chloride hexahydrate, 0.03 M calcium chloride dihydrate, 12.5% (v/v) MPD, 12.5% (w/v) PEG 1000 and 12.5% (w/v) PEG 3350 |
