9G1Y
Staphycoccus aureus MazF in complex with Nabobody 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.830, 59.540, 72.010 |
| Unit cell angles | 90.00, 103.39, 90.00 |
Refinement procedure
| Resolution | 45.367 - 2.697 |
| R-factor | 0.218677503083 |
| Rwork | 0.216 |
| R-free | 0.27434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.367 | 2.860 |
| High resolution limit [Å] | 2.697 | 2.697 |
| Number of reflections | 14597 | 2309 |
| <I/σ(I)> | 4.49 | |
| Completeness [%] | 97.5 | 96.5 |
| Redundancy | 2.67 | 2.67 |
| CC(1/2) | 0.969 | 0.408 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.2 M MgCl2, 0.1 M TRIS-HCl pH 8.5, 30% w/v PEG4000 |
