9G19

Structure of the Nothoceros aenigmaticus LFY DNA-binding domain bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.966
Spacegroup name	P 21 21 2
Unit cell lengths	71.708, 154.104, 53.062
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.002 - 3.092
Rwork	0.243
R-free	0.30310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.700	3.200
High resolution limit [Å]	3.090	3.090
Rmerge	0.106	1.438
Rmeas	0.118	1.591
Rpim	0.051	0.670
Total number of observations	59477	5087
Number of reflections	11139	952
<I/σ(I)>	7.4	0.8
Completeness [%]	98.0
Redundancy	5.3	5.3
CC(1/2)	0.998	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	0.2M lithium chloride, 20% PEG 3350