9G17
Structure of PslG with a covalently- bound pentasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-06 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.95392 |
| Spacegroup name | P 65 |
| Unit cell lengths | 97.692, 97.692, 119.775 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.604 - 1.550 |
| Rwork | 0.142 |
| R-free | 0.17390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.738 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.82)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.604 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.155 | |
| Rpim | 0.057 | 0.926 |
| Number of reflections | 93736 | 4675 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 16.2 | 16.2 |
| CC(1/2) | 0.999 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6 | 277 | 20 mM MES pH 6.0, 50 mM sodium chloride |
