9G0L
Crystal structure of the RING-ZnF1 fragment of SIAH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.850, 69.850, 62.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.660 - 1.900 |
| R-factor | 0.2038 |
| Rwork | 0.202 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.660 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.104 | 2.909 |
| Number of reflections | 12780 | 804 |
| <I/σ(I)> | 17 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.1 | 27.3 |
| CC(1/2) | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | MES-imidazole buffer, PEG550MME-20K, 1,6-hexanediol, 1-butanol, 1,2-propanediol, 2-propanol, 1,4-butanediol, 1,3-propanediol |
