9FZX
Rhizobium phage ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979839 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.039, 100.392, 107.692 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.462 - 2.196 |
| R-factor | 0.2063 |
| Rwork | 0.205 |
| R-free | 0.24640 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.548 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.196 | 2.200 |
| Number of reflections | 72435 | 11524 |
| <I/σ(I)> | 19.56 | |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 13 | |
| CC(1/2) | 0.990 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 24% PEG 4000 100 mM Bis-Tris pH 5.5 |
