9FZD
Structure of OmpA-long in complex with nanobody Nb39
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 52.398, 52.572, 256.746 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.800 - 2.300 |
R-factor | 0.2569 |
Rwork | 0.256 |
R-free | 0.28290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.797 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.800 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.071 | 0.898 |
Rmeas | 0.074 | 0.933 |
Rpim | 0.020 | 0.252 |
Number of reflections | 16264 | 1601 |
<I/σ(I)> | 22.03 | 3.17 |
Completeness [%] | 99.6 | 99.94 |
Redundancy | 13.2 | 13.5 |
CC(1/2) | 0.999 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M sodium chloride, 0.1 M sodium citrate, 12 % PEG 4K, 0.1 M LiSO4 |