9FY8
L2A5 Fab in complex with STn-Thr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.331, 128.144, 98.896 |
| Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
| Resolution | 53.610 - 2.620 |
| R-factor | 0.2505 |
| Rwork | 0.248 |
| R-free | 0.30630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.525 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5109: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.892 | 5.320 | 3.080 |
| High resolution limit [Å] | 2.620 | 3.770 | 2.670 |
| Rmerge | 0.200 | ||
| Rmeas | 0.302 | 0.127 | 0.460 |
| Number of reflections | 34129 | 7204 | 4136 |
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 97.6 | ||
| Redundancy | 5.13 | ||
| CC(1/2) | 0.961 | 0.986 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.1M BisTris:Hcl, pH6.5, 25% (w/v) PEG 3350 |
