9FY5
CRYSTAL STRUCTURE OF HUMAN MONOACYLGLYCEROL LIPASE WITH COMPOUND
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99996 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.916, 127.239, 62.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.960 - 1.430 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.786 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.430 | 1.530 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.100 | 0.920 |
| Number of reflections | 66499 | 12081 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.26 | |
| CC(1/2) | 0.998 | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES pH 6.5, 13% PEG MME5K, 8% 1-propanol |
