9FXQ

Ancestral Prenylcysteine Oxidase 1 (PCYOX1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.969
Spacegroup name	P 41 3 2
Unit cell lengths	172.659, 172.659, 172.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.390 - 3.100
R-factor	0.23015
Rwork	0.225
R-free	0.27546
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.993
Data reduction software	XDS
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	172.770	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.180
Rmeas	0.190
Rpim	0.030
Number of reflections	16624	2936
<I/σ(I)>	15.3	1.56
Completeness [%]	100.0	100
Redundancy	57.6	26.7
CC(1/2)	1.000	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2.0 M Ammonium Sulfate, 0.1 M HEPES, 5% Isopropanol, pH 6.5