9FXN

Human mitochondrial nuclease EXOG (hEXOG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03326
Spacegroup name	P 1 21 1
Unit cell lengths	73.550, 83.560, 74.390
Unit cell angles	90.00, 113.51, 90.00

Refinement procedure

Resolution	40.550 - 1.620
R-factor	0.1587
Rwork	0.157
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.842
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.550	1.720
High resolution limit [Å]	1.620	1.620
Number of reflections	97632	97632
<I/σ(I)>	1.01	1.01
Completeness [%]	93.0	93
Redundancy	6.64
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	16.5 % PEG 8000, 0.1 M Tris-HCL, 0.3 M magnesium chloride hexahydrate