9FWY

Structure of the Nothoceros aenigmaticus LFY DNA-binding domain bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	43.220, 90.900, 152.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.000 - 2.900
R-factor	0.259
Rwork	0.257
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (v5.8)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.000	78.000	2.410
High resolution limit [Å]	2.350	10.490	2.350
Rmerge	0.147	0.028	2.098
Rmeas	0.181	0.035	2.617
Number of reflections	24681	297	1598
<I/σ(I)>	4.9
Completeness [%]	95.2
Redundancy	2.65
CC(1/2)	0.996	0.999	0.204

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	50 mM sodium acetate, pH 4.6, 8% PEG3350