9FWW
Human NKp30 in complex with a VHH variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-23 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 156.457, 156.457, 89.357 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.590 - 1.844 |
| R-factor | 0.1996 |
| Rwork | 0.199 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (23-JAN-2024)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.594 | 2.046 |
| High resolution limit [Å] | 1.844 | 1.844 |
| Rmeas | 0.117 | |
| Rpim | 0.032 | 0.462 |
| Number of reflections | 29007 | 1450 |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 91.3 | |
| Redundancy | 13.5 | |
| CC(1/2) | 0.983 | 0.785 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (v/v) PEG 4000, 20% (v/v) Isopropanol, 0.1 M Sodium citrate pH 5.6 |
