9FU1
Serial microseconds crystallography at ID29 using fixed-target (large foils): Proteinase K with 50 um spacing between X-ray pulses
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2024-03-08 |
| Detector | PSI JUNGFRAU 4M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.690, 68.690, 108.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.100 - 2.000 |
| R-factor | 0.1864 |
| Rwork | 0.182 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.562 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.130 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 18350 | 896 |
| <I/σ(I)> | 3.33 | |
| Completeness [%] | 100.0 | |
| Redundancy | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.5 | 293 | 0.1 M MES pH 6.5, 0.5 M sodium nitrate, 0.1 M calcium chloride |
