9FTW
Crystal structure of calcium-activated EndoU
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 |
Unit cell lengths | 47.723, 49.742, 60.946 |
Unit cell angles | 88.62, 87.49, 70.35 |
Refinement procedure
Resolution | 23.700 - 1.650 |
R-factor | 0.1895 |
Rwork | 0.188 |
R-free | 0.21590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (23-JAN-2024)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 1.670 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 59530 | 8577 |
<I/σ(I)> | 8.81 | |
Completeness [%] | 93.9 | 84.3 |
Redundancy | 1.92 | |
CC(1/2) | 0.996 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M Na Acetate 0.1 M HEPES pH 7.5 22 % PEG 4000 |