9FTW
Crystal structure of calcium-activated EndoU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.723, 49.742, 60.946 |
| Unit cell angles | 88.62, 87.49, 70.35 |
Refinement procedure
| Resolution | 23.700 - 1.650 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (23-JAN-2024)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.670 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 59530 | 8577 |
| <I/σ(I)> | 8.81 | |
| Completeness [%] | 93.9 | 84.3 |
| Redundancy | 1.92 | |
| CC(1/2) | 0.996 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M Na Acetate 0.1 M HEPES pH 7.5 22 % PEG 4000 |
