9FTU

Serial microseconds crystallography at ID29 using ASU extruder: Thaumatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	293
Detector technology	PIXEL
Collection date	2023-04-12
Detector	PSI JUNGFRAU 4M
Wavelength(s)	1.072
Spacegroup name	P 41 21 2
Unit cell lengths	58.550, 58.550, 151.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.320 - 1.750
R-factor	0.1403
Rwork	0.138
R-free	0.16620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.234
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.560	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	27591	1349
<I/σ(I)>	18.82
Completeness [%]	100.0	100
Redundancy	1420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	293	0.1 M HEPES pH 7.5, 1.8 M Sodium Potassium Tartrate