9FTS
Serial microseconds crystallography at ID29 using SACLA extruder: Thaumatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2023-04-13 |
| Detector | PSI JUNGFRAU 4M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.480, 58.480, 151.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.550 - 1.750 |
| R-factor | 0.1538 |
| Rwork | 0.152 |
| R-free | 0.18160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.262 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.480 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 27498 | 1269 |
| <I/σ(I)> | 9.29 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 595 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 293 | 0.1 M HEPES pH 7.5, 1.8 M Sodium Potassium Tartrate |
