9FTR

Drosophila golgi alpha-mannosidase II (dGMII) in complex with amide modified swainsonine-configured alkyl indolizidine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	89.113, 91.939, 133.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.681 - 2.140
Rwork	0.179
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.580
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.681	2.200
High resolution limit [Å]	2.140	2.140
Rmerge	0.045	0.352
Rpim	0.045	0.352
Number of reflections	61056	4448
<I/σ(I)>	9.6	1
Completeness [%]	100.0	100
Redundancy	1.9
CC(1/2)	0.997	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM succinate pH 7.0 and 6-12% (w/v) PEG 3350