9FSW
ClpP from Staphylococcus epidermidis with glycerol in some of the catalytic sites.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.83 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.606, 123.989, 126.688 |
| Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
| Resolution | 63.330 - 2.390 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.732 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.603 | 2.157 |
| High resolution limit [Å] | 1.994 | 1.994 |
| Rmerge | 0.108 | 1.303 |
| Rmeas | 0.117 | 1.408 |
| Rpim | 0.046 | 0.529 |
| Total number of observations | 943499 | 51337 |
| Number of reflections | 148087 | 7294 |
| <I/σ(I)> | 9.4 | 1.5 |
| Completeness [%] | 75.4 | |
| Redundancy | 6.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298.15 | 0.1M sodium acetate (NaOAc), 30 - 40% v/v MPD, and 0.01 - 0.04 M calcium chloride (CaCl2). |
