9FSK
Crystal structure of the HECT domain of Smurf1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.157, 74.318, 161.063 |
| Unit cell angles | 90.00, 111.93, 90.00 |
Refinement procedure
| Resolution | 78.920 - 2.750 |
| R-factor | 0.2512 |
| Rwork | 0.248 |
| R-free | 0.30130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.920 | 2.770 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.092 | 0.600 |
| Number of reflections | 48367 | 2419 |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 98.9 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25 % PEG 3350, 0.1 M Bis Tris pH 5.9, 0.2 M MgCl2, 0.02 M NH4OAc |
