9FPP
FGD2 (Rv0132c) from Mycobacterium tuberculosis with cofactor F420 crystallised with Anderson-Evans polyoxotungstate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2021-11-02 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.310, 89.311, 155.634 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.820 - 2.350 |
| Rwork | 0.210 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.889 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.820 | 2.420 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.282 | 1.040 |
| Rmeas | 0.293 | 1.088 |
| Rpim | 0.080 | 0.317 |
| Number of reflections | 51484 | 4397 |
| <I/σ(I)> | 6.2 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 11.5 |
| CC(1/2) | 0.996 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 0.12M Monosaccharides, 0.1M buffer system 1 pH 6.5 (MES imidazole), 37.5% precipitant mix 4 Morpheus condition F4 |
