9FPC
Architectural insights into the corrin and metal binding sites of CobN cobaltochelatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.130, 74.190, 85.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.143 - 1.965 |
| Rwork | 0.173 |
| R-free | 0.22880 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.196 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUCCANEER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.930 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.046 | 0.339 |
| Number of reflections | 111314 | 4148 |
| <I/σ(I)> | 16.4 | 2.3 |
| Completeness [%] | 98.2 | 86 |
| Redundancy | 3.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2 M Lithium sulfate, 30% polyethylene glycol 4000 and 0.1M Tris pH 8.5 |
