9FP8

Crystal structure of Anopheles gambiae actin depolymerizing factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	273
Detector technology	PIXEL
Collection date	2016-02-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.928
Spacegroup name	P 2 21 21
Unit cell lengths	56.014, 62.423, 87.272
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.140 - 1.680
R-factor	0.2992
Rwork	0.297
R-free	0.33820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.214
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.140	1.740
High resolution limit [Å]	1.680	1.680
Number of reflections	444998	3067
<I/σ(I)>	3.77
Completeness [%]	98.5
Redundancy	6.5
CC(1/2)	0.990	0.294

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.17 M ammonium sulphate, 25.5% w/v polyethylene glycol 4000, 15% v/v glycerol