9FOI

Structure of human KCTD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	67.558, 96.634, 115.995
Unit cell angles	90.00, 98.09, 90.00

Refinement procedure

Resolution	66.890 - 2.710
R-factor	0.2087
Rwork	0.207
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.714
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	114.840	2.850
High resolution limit [Å]	2.710	2.710
Number of reflections	40432	5875
<I/σ(I)>	7	1.1
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.993	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277.15	0.1 M SPG pH 6.0 (mix ratio 2:7:7 succinate/sodium dihydrogen phosphate/glycine) and 60.0% MPD. Cryoprotected with mother liquor supplemented with 25% ethylene glycol.