9FOC
Crystal structure of the PWWP1 domain of NSD2 bound by compound 11.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.630, 67.960, 159.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.618 |
| R-factor | 0.2166 |
| Rwork | 0.214 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (8-JUN-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 159.690 | 1.817 |
| High resolution limit [Å] | 1.616 | 1.616 |
| Rmerge | 0.165 | 1.460 |
| Rmeas | 0.172 | 1.533 |
| Rpim | 0.048 | 0.454 |
| Total number of observations | 370342 | 15748 |
| Number of reflections | 28928 | 1446 |
| <I/σ(I)> | 10.5 | 1.7 |
| Completeness [%] | 70.2 | |
| Redundancy | 12.8 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus, G1. Molecular Dimensions catalogue no.: MD1-47. |
