9FNK
The structure of CBM42 from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-27 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.453, 77.396, 78.769 |
| Unit cell angles | 92.64, 110.06, 102.69 |
Refinement procedure
| Resolution | 37.220 - 2.090 |
| R-factor | 0.2442 |
| Rwork | 0.243 |
| R-free | 0.28670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.929 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.220 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.110 | 0.560 |
| Rmeas | 0.160 | 0.800 |
| Rpim | 0.110 | 0.560 |
| Number of reflections | 67664 | 3863 |
| <I/σ(I)> | 4.7 | 1.4 |
| Completeness [%] | 97.2 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.980 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.0M Ammonium sulfate and 0.1M MES buffer at pH 6.5 |
