9FNH
The glycoside hydrolase family 71 (GH71) member AnGH71C from Aspergillus nidulans in complex with nigerotetraose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.273, 82.242, 87.069 |
| Unit cell angles | 103.52, 105.64, 117.59 |
Refinement procedure
| Resolution | 41.700 - 1.920 |
| R-factor | 0.1693 |
| Rwork | 0.165 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 2.116 |
| High resolution limit [Å] | 1.916 | 1.916 |
| Rmerge | 0.136 | 0.844 |
| Number of reflections | 82756 | 4138 |
| <I/σ(I)> | 6.4 | 1.6 |
| Completeness [%] | 92.1 | 65.5 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.990 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.1 M Citrate pH 5.0 with 20 % w/v PEG 6000 |
