9FNF
The glycoside hydrolase family 71 (GH71) member AnGH71C from Aspergillus nidulans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.873130 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.194, 84.745, 103.322 |
| Unit cell angles | 90.00, 130.85, 90.00 |
Refinement procedure
| Resolution | 39.080 - 1.330 |
| R-factor | 0.1601 |
| Rwork | 0.159 |
| R-free | 0.17630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.080 | 1.350 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.087 | 1.014 |
| Number of reflections | 397430 | 11634 |
| <I/σ(I)> | 7.62 | 0.85 |
| Completeness [%] | 99.5 | 89.33 |
| Redundancy | 3.3 | 2.5 |
| CC(1/2) | 0.997 | 0.314 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M BIS-Tris pH 5.5 with 0.2 M ammonium sulphate and 25 % w/v PEG 3350 |
