9FN6
Full-length crystal structure of human Fascin 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 114.566, 70.804, 122.210 |
| Unit cell angles | 90.00, 92.78, 90.00 |
Refinement procedure
| Resolution | 43.420 - 2.200 |
| R-factor | 0.23747 |
| Rwork | 0.234 |
| R-free | 0.30435 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.420 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.106 | 0.541 |
| Number of reflections | 47005 | 47005 |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0,1M HEPES pH 7.5, 20% polyethylene glycol (PEG) 4000, 2% propanol |
