9FN5
The REC domain (in the non-phosphorylated state) of XynC, a response regulator from G.proteiniphilus T-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-08-27 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 125.737, 125.737, 39.087 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.790 - 2.350 |
| R-factor | 0.1846 |
| Rwork | 0.181 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.037 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.790 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.110 | 0.420 |
| Rmeas | 0.140 | 0.570 |
| Rpim | 0.090 | 0.390 |
| Number of reflections | 9500 | 843 |
| <I/σ(I)> | 5.1 | 2 |
| Completeness [%] | 98.7 | |
| Redundancy | 2.1 | |
| CC(1/2) | 0.980 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 26% PEG 3350 and 0.15M Calcium acetate |
